Site Directed Mutagenesis (SDM) Rat Deletion H9C2

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

Get tips on using TIMP2 (D18B7) Rabbit mAb #5738 to perform Western blotting TIMP-2

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Atg5 (D5G3) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - K562 cells

Get tips on using LAMP1 (C54H11) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y

Get tips on using GABARAP (E1J4E) Rabbit mAb to perform Autophagy assay cell type - HEK 293

Get tips on using Atg16L1 (D6D5) Rabbit mAb to perform Autophagy assay cell type - HEK 293

Get tips on using mTOR (7C10) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y