Get tips on using LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells to perform Live / Dead assay mammalian cells - K562
When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
Get tips on using FastLane Cell Multiplex Kit (200) to perform PCR Multiplex PCR - Mammalian DNA
Get tips on using BEBMTM Bronchial Epithelial Cell Growth Basal Medium to perform Mammalian cell culture media HBEpC
Get tips on using LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit to perform Live / Dead assay mammalian cells - mouse keratinocytes
Get tips on using CellGenix® GMP SCGM Stem Cell Growth Medium to perform Mammalian cell culture media NK-92
Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.
Get tips on using Corning® Transwell® polycarbonate membrane cell culture inserts to perform Cell migration / Invasion cell type - RAW 264.7
Get tips on using In Situ Cell Death Detection Kit, TMR red to perform TUNEL assay cell type - Rabbit synovial fibroblasts
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