siRNA / miRNA gene silencing Human Calu-3

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Get tips on using peSpCas9(1.1)-2×sgRNA (empty, donor) to perform CRISPR Human - Repression SLC7A11

Products Addgene peSpCas9(1.1)-2×sgRNA (empty, donor)
CDX2 Product

Get tips on using CDX2 to perform Immunohistochemistry Human - CDX2

Products BIOCARE MEDICAL CDX2

Get tips on using 53BP1 Antibody (H-300) to perform TissueFAxs 53BP1 [H-300] - Rabbit Human -NA-

Products Santa Cruz Biotechnology 53BP1 Antibody (H-300)

Get tips on using 53BP1 Antibody (H-300) to perform Immunofluorscence  53BP1 [H-300] - Rabbit Human -NA-

Products Santa Cruz Biotechnology 53BP1 Antibody (H-300)

Get tips on using REPLI-g Mitochondrial DNA Kit (25) to perform Whole Genome Amplification Human

Products Qiagen REPLI-g Mitochondrial DNA Kit (25)

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells immortalized MDA-MB-361

Get tips on using Advanced DMEM to perform 3D Cell Culture Media Human cancer esophageal organoids

Products Thermo Fisher Scientific Advanced DMEM
DMEM/F-12 Product

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Human gastric organoids

Products Thermo Fisher Scientific DMEM/F-12

Get tips on using Keratinocyte SFM (1X) to perform 3D Cell Culture Media Human prostate organoid

Products Thermo Fisher Scientific Keratinocyte SFM (1X)
DMEM/F-12 Product

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Human endometrium organoids

Products Thermo Fisher Scientific DMEM/F-12

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