Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?
Get tips on using LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation to perform Live / Dead assay mammalian cells - rat testicular tissue
Get tips on using Tetro cDNA Synthesis Kit to perform cDNA synthesis Tissue
Get tips on using qScript cDNA Synthesis Kit to perform cDNA synthesis Tissue
Get tips on using QuantiTect Reverse Transcription Kit to perform cDNA synthesis Tissue
Get tips on using iScript cDNA Synthesis Kit to perform cDNA synthesis Tissue
Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Rat - Gingival tissue
Get tips on using miScript II RT Kit (50) to perform cDNA synthesis Tissue
Get tips on using First-Strand cDNA Synthesis Kit to perform cDNA synthesis Tissue
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment