siRNA / miRNA gene silencing Human MCF-7

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Whole genome profiling - OVCAR-3 human ovarian cancer

Get tips on using EpiQuik Dnmt3A Assay Kit to perform DNA methylation profiling Whole genome profiling - human peripheral blood mononuclear cells

Get tips on using MethylFlash Hydroxymethylated DNA Quantification Kit to perform DNA methylation profiling Whole genome profiling - human germ cell cancer

Get tips on using EZ-96 DNA Methylation-Gold™ Kit to perform DNA methylation profiling Whole genome profiling - human placenta

Get tips on using SENSE mRNA-Seq Library Prep Kit V2 to perform RNA sequencing Human - Glioblastoma stem-like cells (GSCs)

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Get tips on using ISOGEN to perform RNA isolation / purification Cells - primary human epidermal melanocytes

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Primary cells Human chondrocytes

Get tips on using Advanced DMEM to perform 3D Cell Culture Media Human esophageal organoids

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - Human lung fibroblasts