siRNA / miRNA gene silencing Human SPC-A1

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Human Spleen

Get tips on using XcelGen Blood gDNA Mini Kit to perform Whole Genome Amplification Human

Get tips on using EpiTect Whole Bisulfitome Kit (25) to perform Whole Genome Amplification Human

Get tips on using REPLI-g FFPE Kit (100) to perform Whole Genome Amplification Human

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

I work with Human liver organoids and I would like to know the impact of FBS on the organoids. Since the composition of FBS is unknown, would you recommend any alternatives such as human platelet lysate?

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - MIA PaCa-2

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - MDA-MB-231

Get tips on using KAPA Stranded mRNA-Seq Kit to perform RNA sequencing Human - PBMCs

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Spinal cord