CRISPR Rat Deletion INS-1 832/13

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Mouse - Deletion B16-F1 PC7

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Mouse - Deletion B16-F1 Furin

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Mouse - Deletion B16-F1 β2m

Get tips on using pSPCas9(BB)-2A-GFP plasmids to perform CRISPR Mouse - Deletion 3T3-L1 Cep350

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Mouse - Deletion 3T3-L1 MmP13

Get tips on using pSPCas9(BB)-2A-GFP plasmids to perform CRISPR Mouse - Deletion 3T3-L1 Usp2

Get tips on using pSpCas9(BB)-2A-Puro (PX459) V2.0 to perform CRISPR Mouse - Deletion ATDC5 MEK1

Get tips on using IFITM1 CRISPR Activation Plasmid (h) to perform CRISPR Human - Activation IFITM1

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using pLKO.1 puro to perform CRISPR Mouse - Activation RAW 264.7 Dmd