When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Uterus
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Hypothalamus
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Tonsil
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human testes
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Tendons
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Prostate
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Placenta
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Pancreas
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