Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Get tips on using Cell Counting Kit-8 to perform Cell cytotoxicity / Proliferation assay cell type - HT22 mouse hippocampal cells
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Get tips on using Gibco™DMEM, low glucose, pyruvate to perform Stem cell culture media Human bone mesenchymal stem cell (BMSC)
Get tips on using FlowCellect Autophagy LC3 antibody based kit to perform Autophagy assay cell type - MT-2 (human T cell leukaemia)
Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - MT-2 (human T cell leukaemia)
Get tips on using Cell Counting Kit-8 to perform Live / Dead assay mammalian cells - rat nucleus pulposus
Get tips on using Monoclonal Mouse Anti-Human Cytokeratin 7 (Dako Omnis) Clone OV-TL 12/30 to perform Immunohistochemistry Human - CK7
Get tips on using Cell Counting Kit-8 to perform Live / Dead assay mammalian cells - INS-1 832/12
Get tips on using Endothelial Cell Medium to perform Mammalian cell culture media CADMEC/HMVEC
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment