siRNA / miRNA gene silencing Human Melanoma cells (501 Mel and SK Mel 28)

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using pGL3-Basic Vector to perform Reporter gene assay luciferase - SW480 human colon cancer cell line

Get tips on using EasySep™ Human Cord Blood CD34 Positive Selection Kit II to perform Cell Isolation CD34+ cells

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using AquaRNA Kit to perform RNA isolation / purification Cells - primary human mesenchymal stem cells

Get tips on using PE anti-human CD96 (TACTILE) Antibody to perform Flow cytometry Anti-bodies Human - CD96

Get tips on using PE/Cyanine7 anti-human CD14 Antibody to perform Flow cytometry Anti-bodies Human - CD14

Get tips on using PerCP/Cyanine5.5 anti-human CD49d Antibody to perform Flow cytometry Anti-bodies Human - CD49d

Get tips on using PerCP/Cyanine5.5 anti-human CD4 Antibody to perform Flow cytometry Anti-bodies Human - CD4

Get tips on using CD11b Antibody, anti-human/mouse, FITC to perform Flow cytometry Anti-bodies Human - CD11b