DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.
Get tips on using MouseTRAP™ (TRAcP 5b) ELISA to perform Acid phosphatase assay cell type - murine macrophage cells
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Human Kidney
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Human Liver
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Stomach
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Spleen
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Rat Nose
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Lung
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Liver
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