siRNA / RNAi /miRNA transfection Human Cal 27 cells

Get tips on using Human Chitinase 3-like 1/YKL-40 PicoKine™ ELISA Kit EK0974 to perform ELISA Human - Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human dermal fibroblasts

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human osteoblasts - osteoarthritis

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human lung fibroblasts

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human epidermal melanocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human epidermal keratinocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human chondrocytes - osteoarthritis

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized Human blood cord

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human preadipocytes