siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1)

Get tips on using IRIS9 Plus Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using iBright™ Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using CozyHi™ Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using CozyXL™ Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using Cozy™ Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using pRNAT-H1.1/Neo to perform shRNA gene silencing Mouse - 4T1 Integrin α6

Get tips on using EZQ™ Protein Quantitation Kit to perform Protein quantification Fluorimetric method

Get tips on using Qubit™ Protein Assay Kit to perform Protein quantification Fluorimetric method

Get tips on using NanoOrange™ Protein Quantitation Kit to perform Protein quantification Fluorimetric method

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.