DNA methylation profiling Gene specific profiling Rat whole pituitary glands

Get tips on using siRNA EGFR to perform siRNA / miRNA gene silencing Human - A431 EGFR

Get tips on using Cnp siRNA to perform siRNA / miRNA gene silencing Mouse - BV2 CNPase

Get tips on using Wwtr1 siRNA to perform siRNA / miRNA gene silencing Mouse - C2C12 Taz

Get tips on using Bambi siRNA to perform siRNA / miRNA gene silencing Mouse - C2C12 BAMBI

Get tips on using Stk11 siRNA to perform siRNA / miRNA gene silencing Mouse - C2C12 Stk11

Get tips on using Cxcr4 siRNA to perform siRNA / miRNA gene silencing Mouse - C2C12 CXCR4

Get tips on using Dusp3 siRNA to perform siRNA / miRNA gene silencing Mouse - BMDMs Dusp3

Get tips on using AllStars Hs Cell Death siRNA to perform siRNA / miRNA gene silencing Human - U2OS KRAS

Get tips on using PACE4 siRNA (h) to perform siRNA / miRNA gene silencing Human - DuCaP

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.