rna-isolation-purification-cells-immortalized-mda-mb-231

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Human - Huh7

Get tips on using Ovation® RNA-Seq System V2 to perform RNA sequencing Human - H7

Get tips on using TruSeq Small RNA Library Preparation Kit to perform RNA sequencing Human - HEK293T

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Human - HEK293T

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Human - HeLa

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - BHK-21

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - Rat_Mesenteric fat

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Mouse - NSC-34

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Mouse - BV-2