siRNA / miRNA gene silencing Human SPC-A1

Get tips on using EZ-ChIP™ to perform ChIP Human - HUVEC

Get tips on using SCGB1A1 antibody (Secretoglobin, Family 1A, Member 1 (Uteroglobin)) (Middle Region) to perform Immunohistochemistry Human - SCGB1A1 /CC10

Get tips on using REALTOTAL RNA Spin Plus to perform RNA isolation / purification Cells - primary human aortic smooth muscle cells

Plasmid isolation is an important technique in molecular biology or any kind of genetic editing. It involves amplifying plasmids overnight by transforming them into competent bacterial cells. The desired colonies of these bacteria can then be grown in shaker cultures, at appropriate shaking speed, oxygen availability and temperature. These liquid cultures can then be ultracentrifuged to pellet the bacteria, which are then used for plasmid isolation. The bacteria are first resuspended in a buffer, then lysed, neutralized, purified in a column, eluted, precipitated with ethanol and then resuspended. During plasmid isolation, it is important to lyse cells quickly because lysing bacteria for too long may lead to irreversible denaturing of the plasmid. Usually, alkaline lysis is used for isolation because it is a mild treatment. It isolates plasmid DNA and other cell components such as proteins by breaking cells apart with an alkaline solution. Precipitation removes the proteins, and the plasmid DNA recovers with alcohol precipitation. Resuspension and lysis buffers should be mixed thoroughly in order to prevent the DNA from breaking into smaller fragments. This is because broken gDNA can reanneal and remain in the solution, without binding to the column.

Get tips on using MethylCap kit to perform DNA methylation profiling Whole genome profiling - DU145, PC3 human prostate cancer

Get tips on using CC10 Antibody (E-11): sc-365992 to perform Immunohistochemistry Human - SCGB1A1 /CC10

Get tips on using CC10 Antibody (B-6): sc-390313 to perform Immunohistochemistry Human - SCGB1A1 /CC10

Get tips on using Unrestricted HD-CGH Microarray ISCA v2, 4x44k to perform Microarray Comperative genomic hybridization - Human PBMCs

Get tips on using ScriptSeq Complete Gold Kit (Epidemiology) to perform RNA sequencing Human - SH-SY5Y

Get tips on using FGF-10 Antibody (3C7): sc-293208 to perform Immunohistochemistry Human - FGF-10