siRNA / RNAi /miRNA transfection Human Cells OV-2008

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Cells - primary human osteoblasts

Get tips on using Cytoselect™ Cell Viability and Cytotoxicity Assay to perform Live / Dead assay mammalian cells - MDA-MB-231 human breast cancer cells

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell Differentiation media human umbilical mesenchymal stem cells (hUMSCs) differentiation into osteogenic cells

Get tips on using Ovation® RNA Amplification System V2 to perform Microarray RNA amplification & Labeling - Human endometrial stromal cells Cyanine 3-pCp

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using SENSE mRNA-Seq Library Prep Kit V2 to perform RNA sequencing Human - Glioblastoma stem-like cells (GSCs)

Get tips on using MitoSOX™ Red Mitochondrial Superoxide Indicator, for live-cell imaging to perform ROS assay cell type - A549 human adenocarcinomic human alveolar basal epithelial cells

Get tips on using RNAqueous™ Total RNA Isolation Kit to perform RNA isolation / purification Cells - primary human lung fibroblasts

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.