ChIP acH4 Donkey Chicken

Get tips on using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads) #9004 to perform ChIP Mouse - Panc02

Get tips on using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads) #9004 to perform ChIP Mouse - BMDCs

Get tips on using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 to perform ChIP Human - Glioblastoma cell line

Get tips on using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 to perform ChIP Mouse - 3T3-L1 cells

Get tips on using Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonal to perform ChIP Anti-bodies H3K4me3

Get tips on using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005 to perform ChIP Rat - Mesenteric arteries

Get tips on using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads) #9004 to perform ChIP Human - PANC-1

Get tips on using Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb #9728 to perform ChIP Anti-bodies H3K27me2

Get tips on using Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit mAb #5326 to perform ChIP Anti-bodies H3K4me1

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.