siRNA / miRNA gene silencing Human SKOV-3

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Human lung cancer organoids

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Human cancer colon organoids

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Human small intestinal organoids

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Fenozol to perform RNA isolation / purification Tissue - human aorta tissue

Get tips on using Anti-Collagen Type VII Antibody, clone 32,-VII to perform Immunohistochemistry Collagen VII [II-32] - Mouse Human -NA-

Get tips on using CD20 V450 to perform Flow cytometry Anti-bodies Human - CD20

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Placenta

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Lung

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Vagina