dna-isolation-purification-cells-primary-cells-mouse-embryonic-fibroblast-mef

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using Zombie Violet™ Fixable Viability Kit to perform Live / Dead assay mammalian cells - mouse microglia

Get tips on using Zombie UV™ Fixable Viability Kit to perform Live / Dead assay mammalian cells - mouse splenocytes

Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - Caco-2

Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - SH-SY5Y

Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - BHK-21

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Immortalized cell lines PANC-1

Get tips on using NeuroMag to perform DNA transfection Mammalian cells - Immortalized cell lines SH-SY5Y

Get tips on using Purified Rat Anti-Mouse CD31 to perform Immunohistochemistry Mouse - CD31

Get tips on using Biotin Rat Anti-Mouse CD24 to perform Immunohistochemistry Mouse - CD24