Site Directed Mutagenesis (SDM) Human Point mutation THP-1

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Human pancreatic cancer organoids

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Human cancer esophageal organoids

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Human lung cancer organoids

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Human cancer colon organoids

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media Human salivary gland stem cells

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using CD206 (MMR) Monoclonal Antibody (19.2), PE, eBioscience™ to perform Flow cytometry Anti-bodies Human - CD206

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media Human Tendon Stem/Pluripotence cells (TSPCs)

Get tips on using Purified Mouse Anti-β-Catenin Clone 14/Beta-Catenin (RUO) to perform Immunohistochemistry Human - β-catenin