dna-isolation-purification-cells-primary-cells-mouse-embryonic-fibroblast-mef

Get tips on using Mouse Leptin ELISA Kit (ab100718) to perform ELISA Mouse - Leptin

Get tips on using Mouse GDNF ELISA Kit (ab171178) to perform ELISA Mouse - GDNF

Get tips on using Mouse Fibronectin ELISA Kit (ab108849) to perform ELISA Mouse - Fibronectin

Get tips on using Human/Mouse BDNF DuoSet ELISA to perform ELISA Mouse - BDNF

Get tips on using Mouse Adiponectin/Acrp30 DuoSet ELISA to perform ELISA Mouse - Adiponectin

Get tips on using Mouse Adiponectin ELISA Kit (ab108785) to perform ELISA Mouse - Adiponectin

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - Mouse macrophages

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - Mouse microglia

Get tips on using Quant-iT™ RNA Assay Kit to perform RNA quantification Fuorimetric - mouse glial cells

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.