siRNA / RNAi /miRNA transfection Human Cells NK-92

Get tips on using IGEPAL® CA-630 to perform Protein isolation Mammalian cells - Human lung fibroblasts

Get tips on using NanoOrange™ Protein Quantitation Kit to perform Protein quantification Mammalian cells - Human podocytes

Get tips on using ZR RNA MiniPrepTM kit to perform RNA isolation / purification Cells - primary human islets of langerhans

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - primary human dermal fibroblasts

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Cells - primary human CD14+ monocytes

Get tips on using Quant-iT™ RNA Assay Kit to perform RNA quantification Fuorimetric - human metastatic melanoma cells

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.