siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells

Get tips on using REPLI-g Mitochondrial DNA Kit (25) to perform Whole Genome Amplification Human

Get tips on using Direct RNA Sequencing Kit to perform RNA sequencing Human

Get tips on using REG1 beta Polyclonal Antibody to perform Immunohistochemistry Human - REG1

Get tips on using Anti-Hes1 antibody (ab49170) to perform Immunohistochemistry Human - Hes1

Get tips on using Anti-Notch1 antibody (ab27526) to perform Immunohistochemistry Human - Notch1

Get tips on using MLH1 antibody [G168-15] to perform Immunohistochemistry Human - MLH1

Get tips on using Anti-CRISP3 antibody (ab105951) to perform Immunohistochemistry Human - CRISP3

Get tips on using EpiTect ChIP OneDay Kit to perform ChIP Human - AGS

Get tips on using Re-ChIP-IT® to perform ChIP Human - LCL

Stem cells have the unique ability to self-renew or differentiate themselves into various cell types in response to appropriate signals. These cells are especially important for tissue repair, regeneration, replacement, or in the case of hematopoietic stem cells (HSCs) to differentiate into various myeloid populations. Appropriate signals refer to the growth factor supplements or cytokines that mediate differentiation of various stem cells into the required differentiated form. For instance, HSCs can be differentiated into dendritic cells (with IL-4 and GM-CSF), macrophages (with m-CSF) and MDSCs (with IL-6 and GM-CSF). Human pluripotent stem cells (hPSCs) and induced pluripotent stem cells (iPSCs) can be first cultured in neural differentiation media (GSK3𝛃-i, TGF𝛃-i, AMPK-i, hLIF) to form neural rosettes, which can be differentiated into neural or glial progenitors (finally differentiated into oligodendrocytes). Neural progenitors can be finally differentiated into glutaminergic (dibytyryl cAMP, ascorbic acid) and dopaminergic (SHH, FGF-8, BDNF, GDNF, TGF-𝛃3) neurons. Thus, it is important to first identify the self-renewing cell line: its source and its final differentiation state, followed by the supplements and cytokines required for the differentiation, and final use. Timelines are another thing that is considered. For instance, it takes 7-10 days to form neural rosettes from iPSCs and 3 days to differentiate neural progenitors to neurons. Finally, the stability for stem cell culture media varies. It is advised to make fresh media every time when differentiating HSCs to myeloid populations, whereas neural differentiation media may remain stable for two weeks when stored in dark between 2-8C.