siRNA / miRNA gene silencing Human BCP-1

Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media Human WA09 hESC

Get tips on using Anti-panCytokeratin antibody [C-11] (ab7753) to perform Flow cytometry Anti-bodies Human - Keratin

Get tips on using MethylCap kit to perform DNA methylation profiling Whole genome profiling - DU145, PC3 human prostate cancer

Get tips on using VENTANA anti-MSH2 (G219-1129) Mouse Monoclonal Primary Antibody to perform Immunohistochemistry Human - MSH2

Get tips on using Unrestricted HD-CGH Microarray ISCA v2, 4x44k to perform Microarray Comperative genomic hybridization - Human PBMCs

I work with Human liver organoids and I would like to know the impact of FBS on the organoids. Since the composition of FBS is unknown, would you recommend any alternatives such as human platelet lysate?

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression HBV RT

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Activation α-synuclein

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression miR-21