Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human HUVEC
The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.
Get tips on using Gibco DMEM/F-12, HEPES to perform 3D Cell Culture Media Human primary breast ephitelial cells-organoids
Get tips on using Gibco DMEM/F-12, HEPES to perform Stem cell Differentiation media hiPSC differentiation into Human Neuronal cells
Get tips on using ToxCount™ Cell Viability Assay to perform Live / Dead assay mammalian cells - glioblastoma stem cells
Get tips on using Cell Death Detection ELISA to perform TUNEL assay cell type - Rat pulmonary arterial smooth muscle cells
Get tips on using X-VIVOTM 10 Serum-free Hematopoietic Cell Medium to perform Mammalian cell culture media NK-92
Get tips on using MTT Cell Growth Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - oral squamous cell carcinoma
Get tips on using TaKaRa MiniBEST Universal Genomic DNA Extraction Kit to perform DNA isolation / purification Cells - Immortalized cell lines SH-SY5Y
Get tips on using X-VIVOTM 10 Serum-free Hematopoietic Cell Medium to perform 3D Cell Culture Media Mouse thymic organoids
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