ChIP H3K27me3 Horse Sheep

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ELISA Human - Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40 Experiment

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Proteins ELISA Human Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

ELISA Mouse - Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40 Experiment

Proteins ELISA Mouse Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

MicroVue YKL-40 EIA Product

Get tips on using MicroVue YKL-40 EIA to perform ELISA Human - Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

Products Quidel MicroVue YKL-40 EIA

pT7CFE1-CHis Product

Get tips on using pT7CFE1-CHis to perform Protein Expression Eukaryotic cells - Leukocytes extracts Nluc

Products Govind Rao, Center for Advanced Sensor Technology (CAST) and Dep pT7CFE1-CHis

pX330-U6-Chimeric_BB-CBh-hSpCas9 Product

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Activation RANKL