ChIP acH3 Rat Sheep BEF Tag

Get tips on using Rat VEGF ELISA Kit (ab100786) to perform ELISA Rat - VEGF

Get tips on using Rat VEGF Quantikine ELISA Kit to perform ELISA Rat - VEGF

Get tips on using Rat prolactin,PRL ELISA Kit to perform ELISA Rat - PRL

Get tips on using Rat GDNF ELISA Kit (ab213901) to perform ELISA Rat - GDNF

Get tips on using Rat Fibronectin ELISA Kit (ab108850) to perform ELISA Rat - Fibronectin

Get tips on using Mouse Rat Estradiol ELISA Kit to perform ELISA Rat - Estradiol

Get tips on using Rat CX3CL1/Fractalkine DuoSet ELISA to perform ELISA Rat - CX3CL1

Get tips on using Rat Adiponectin ELISA Kit (ab108784) to perform ELISA Rat - Adiponectin

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Rat Transferrin ELISA Kit (ab137993) to perform ELISA Rat - Transferrin (Tf)