Protein Expression Prokaryotic cells B. subtilis

Get tips on using pBESTExp4:IL-10 to perform Protein Expression Prokaryotic cells - B. bifidum murine IL-10

Get tips on using pBESTBL1181:IL-10 to perform Protein Expression Prokaryotic cells - B. bifidum murine IL-10

Get tips on using pTSara-NatB to perform Protein Expression Prokaryotic cells - E. coli N-terminal acetyltransferase B

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using pPICZ-B to perform Protein Expression Eukaryotic cells - P. pastoris TRAAK

Get tips on using B-PER™ Bacterial Protein Extraction Reagent to perform Protein isolation Bacteria - Listeria monocytogenes

Get tips on using B-PER™ Bacterial Protein Extraction Reagent to perform Protein isolation Bacteria - Streptococcus mutans

Get tips on using B-PER™ Bacterial Protein Extraction Reagent to perform Protein isolation Bacteria - Lactobacillus casei

Get tips on using B-PER™ Bacterial Protein Extraction Reagent to perform Protein isolation Bacteria - Synechocystis sp (6803)_Cyanobacteria