PCR

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Unstained Protein Ladder, Broad Range Ladder to perform Protein Ladder Unstained

Get tips on using SuperSignal™ Molecular Weight Protein Ladder to perform Protein Ladder Unstained

Get tips on using Pierce™ Unstained Protein MW Marker to perform Protein Ladder Unstained

Get tips on using Biotinylated Protein Ladder Detection Pack #7727 to perform Protein Ladder Immunofluorescence

Get tips on using Tau Protein Ladder, 6 isoforms human to perform Protein Ladder Immunofluorescence

Get tips on using PR Antibody (F-4): sc-166169 to perform Immunohistochemistry Human - PR

Get tips on using pUC19/Pr-EC to perform Protein Expression Eukaryotic cells - K. lactis α-l-Fucosidase

Get tips on using Recombinant Anti-PRMT5 antibody [EPR5772] (ab109451) to perform Immunohistochemistry Rat - PRMT5

Get tips on using PR Antibody (F-4): sc-166169 to perform Immunohistochemistry Mouse - PR