Immunohistochemistry Collagen Type VII

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Human Veins

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human umbilical vein smooth muscle cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human umbilical vein endothelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human umbilical vein smooth muscle cells

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Cells - primary human umbilical vein endothelial cells

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - primary human umbilical vein endothelial cells

Get tips on using E.Z.N.A.® Total RNA Kit I to perform RNA isolation / purification Cells - primary bovine umbilical vein endothelial cells