Get tips on using 3D-Gene® Mouse miRNA Oligo chip (ver.21) to perform Microarray Gene expression arrays - Mouse liver tissue Cyanine-3-CTP
TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - Rat_Circumvallate papillae
Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - 3T3-L1
Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - THP-1
Get tips on using Trichloroacetic acid to perform Protein isolation Mammalian cells - THP-1
Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - CHO-K1
Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - HaCaT
Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - HepG2
Get tips on using Nuclear Extract Kit to perform Protein isolation Mammalian cells - HOG
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