siRNA / RNAi /miRNA transfection Human Cells A549

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Human aortic endothelial cells

Get tips on using Cell Death Detection ELISA to perform Apoptosis assay cell type - Human endometrial stromal cells

Get tips on using miRNeasy Serum/Plasma Kit to perform RNA isolation / purification Tissue - Human Blood / Serum / Plasma / Buffy coat

Get tips on using In situ apoptosis detection to perform Apoptosis assay cell type - Human endometrial stromal cells

Get tips on using psiCHECK-2vector to perform Reporter gene assay luciferase - HEK 293 human embryonic kidney cells

Get tips on using pFastBac-MRP4-his6 to perform Protein Expression Eukaryotic cells - S. frugiperda human MRP4-his6