Immunohistochemistry Collagen Type I Goat Mouse

Get tips on using Mem-PER™ Plus Membrane Protein Extraction Kit to perform Protein isolation Mammalian cells - Mouse_Brown fat

Get tips on using Anti-GATA4 Antibody to perform Western blotting GATA-4

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Anti-GATA4 antibody (ab84593) to perform Western blotting GATA-4

Get tips on using CellGenix® GMP SCGM Stem Cell Growth Medium to perform Mammalian cell culture media NK-92

Get tips on using GATA-1 shRNA Plasmids (h) to perform shRNA gene silencing Human - TF‐1 GATA‐1

Get tips on using GATA-4 Antibody (G-4): sc-25310 to perform Western blotting GATA-4

Get tips on using GATA-3 Antibody (HG3-31): sc-268 to perform ChIP Anti-bodies GATA3

Get tips on using Recombinant Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428) to perform ChIP Anti-bodies GATA3