siRNA / miRNA gene silencing Mouse siRNA negative control

Get tips on using CD115 (c-fms) Monoclonal Antibody (AFS98), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD115

Get tips on using CD86 (B7-2) Monoclonal Antibody (GL1), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD86

Get tips on using CD8a Monoclonal Antibody (53-6.7), eFluor 450, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD8a

Get tips on using CD11b Monoclonal Antibody (M1/70), eFluor 450, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD11b

Get tips on using CD45 Monoclonal Antibody (30-F11), PE-Cyanine7, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD45

Get tips on using CellROX™ Deep Red Reagent, for oxidative stress detection to perform ROS assay cell type - mouse splenocytes

Get tips on using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® to perform RNA sequencing Mouse - C2C12

Get tips on using Purified anti-Tubulin β-3 (TUBB3) Antibody (Previously Covance catalog# PRB-435P) to perform Immunohistochemistry Mouse - TUBB3

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.