dna-quantification-human-pc-3

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using MLM3636 to perform CRISPR Human - Activation VEGFA

Get tips on using hCas9 to perform CRISPR Human - Deletion Hsp90α

Get tips on using Progesterone Receptor to perform Immunohistochemistry Human - PR

Get tips on using Estrogen Receptor to perform Immunohistochemistry Human - ER

Get tips on using Cytokeratin 20 to perform Immunohistochemistry Human - CK20

Get tips on using Cytokeratin 7 to perform Immunohistochemistry Human - CK7

Get tips on using Anti-CK7 to perform Immunohistochemistry Human - CK7

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.