Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media Human myogenic progenitor cells
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary human peripheral blood monocytes
Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary human osteoblasts - rheumatoid arthritis
Get tips on using ZR RNA MiniPrepTM kit to perform RNA isolation / purification Cells - primary human islets of langerhans
Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - primary human dermal fibroblasts
Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Cells - primary human CD14+ monocytes
Get tips on using Quant-iT™ RNA Assay Kit to perform RNA quantification Fuorimetric - human metastatic melanoma cells
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