ChIP acH4 Rat Donkey

Get tips on using Rat sE-Selectin ELISA Kit (sCD62E) (ab171334) to perform ELISA Rat - sE-Selectin

Get tips on using Rat/Mouse Cytochrome c Quantikine ELISA Kit to perform ELISA Rat - Cytochrome c

Get tips on using Rat BMP-2 PicoKine™ ELISA Kit to perform ELISA Rat - BMP-2

Get tips on using Rat TGF beta 1 ELISA Kit (ab119558) to perform ELISA Rat - TGF-beta 1

Get tips on using Rat IL-1 beta ELISA Kit (ab100768) to perform ELISA Rat - IL-1 beta

Get tips on using Rat ICAM-1 PicoKine™ ELISA Kit to perform ELISA Rat - ICAM-1/CD54

Get tips on using Rat ICAM-1/CD54 Quantikine ELISA Kit to perform ELISA Rat - ICAM-1/CD54

Get tips on using Rat C-Reactive Protein/CRP DuoSet ELISA to perform ELISA Rat - C-Reactive Protein/CRP

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.