Site Directed Mutagenesis (SDM) Human Point mutation HEK293

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using Luminescent β-galactosidase Detection Kit II to perform Reporter gene assay β-galactosidase substrates - HEK293 human embryonic kidney cells

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - HEK293 human embryonic kidney cells

Get tips on using pJAP2 to perform Protein Expression Eukaryotic cells - HEK293 AT1R

Get tips on using pJAP34 to perform Protein Expression Eukaryotic cells - HEK293 A1R

Get tips on using pJAP37 to perform Protein Expression Eukaryotic cells - HEK293 A1R

Get tips on using TruSeq Small RNA Library Preparation Kit to perform RNA sequencing Human - HEK293T

Get tips on using YFP‐TOP2β to perform Protein Expression Eukaryotic cells - HEK293 TOP2β

Get tips on using YFP‐TOP2α to perform Protein Expression Eukaryotic cells - HEK293 TOP2α

Get tips on using pcDNA-nGP1 to perform Protein Expression Eukaryotic cells - HEK293 GP1