Site Directed Mutagenesis (SDM) Human Point mutation THP-1

Get tips on using Atg3 Antibody to perform Autophagy assay cell type - THP 1

Get tips on using LC3B Antibody to perform Autophagy assay cell type - THP 1

Get tips on using LC3B Antibody to perform Autophagy assay cell type - THP 1

Get tips on using Monoclonal Mouse Anti-Human Ki-67 Antigen (Dako Omnis) Clone MIB-1 to perform Immunohistochemistry Human - Ki-67

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - THP-1

Get tips on using Trichloroacetic acid to perform Protein isolation Mammalian cells - THP-1

Get tips on using SAPK/JNK Antibody to perform Autophagy assay cell type - THP 1

Get tips on using SQSTM1/p62 Antibody to perform Autophagy assay cell type - THP 1

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized THP-1

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.