RNA isolation / purification Tissue Mouse

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DNeasy PowerClean Pro Cleanup Kit (50) Product

Get tips on using DNeasy PowerClean Pro Cleanup Kit (50) to perform DNA isolation / purification Water samples

Products Qiagen DNeasy PowerClean Pro Cleanup Kit (50)

Is a bacterial nuclease contamination possible during protein purification? Discussion

I am currently using a recombinant protein which shows metal-dependent DNase activity. Is it possible to pinpoint the source of the DNase activity after protein purification? More specifically, can I ensure that the DNase activity is not because of nuclease contamination from the E.coli that might have persisted and passed with the protein of interest during purification?

Discussions Is a bacterial nuclease contamination possible during protein purification?

KAPA Stranded mRNA-Seq Kit Product

Get tips on using KAPA Stranded mRNA-Seq Kit to perform RNA sequencing Mouse - Chondrocytes

Products Roche Lifesciences KAPA Stranded mRNA-Seq Kit

Site Directed Mutagenesis (SDM) Mouse - Point mutation 3T3-L1 S6 kinase 1 Experiment

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

DNA Site Directed Mutagenesis (SDM) Mouse Point mutation 3T3-L1 S6 kinase 1

Protein expression and purification Yeast - Pichia pastoris hmPRα Experiment

Proteins Protein expression and purification Yeast Pichia pastoris hmPRα

Protein expression and purification Yeast - Pichia pastoris Chymase Experiment

Proteins Protein expression and purification Yeast Pichia pastoris Chymase

Protein expression and purification Yeast - Saccharomyces cerevisiae ΔHSPA5 Experiment

Proteins Protein expression and purification Yeast Saccharomyces cerevisiae ΔHSPA5

Protein expression and purification Insect cells - Sf9 Drosha Experiment

Proteins Protein expression and purification Insect cells Sf9 Drosha

Protein expression and purification Insect cells - Hi5 TYR Experiment

Proteins Protein expression and purification Insect cells Hi5 TYR

Protein expression and purification Insect cells - S2 HER2 Experiment