RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Bronchi

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Breast

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Bone

Get tips on using RNeasy PowerPlant Kit (50) to perform RNA isolation / purification Plants - Seeds

Get tips on using RNeasy PowerWater Kit (50) to perform RNA isolation / purification Water samples

Get tips on using CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS) to perform Cell cytotoxicity / Proliferation assay cell type - MG-63

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Invitrogen™ Cell Culture Contamination Detection Kit to perform Cell Culture Contamination Detection Kit Bacteria

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - Mouse liver tissue

Get tips on using AllStars Hs Cell Death siRNA to perform siRNA / miRNA gene silencing Human - U2OS KRAS