siRNA / miRNA gene silencing Human Melanoma cells (501 Mel and SK Mel 28)

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary human cardiac fibroblasts

Get tips on using NAPSIN A (TMU-AD02) ANTI-HUMAN MOUSE IGG MOAB to perform Immunohistochemistry Human - Naspsin A

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Human Thrombopoietin R/Tpo R APC-conjugated Antibody to perform Flow cytometry Anti-bodies Human - CD110/Thrombopoietin R