DNA gel extraction / PCR product purification

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized Jurkat

Get tips on using XcelGen Plant RNA Isolation Mini Kit to perform RNA isolation / purification Plants - Seeds

Get tips on using RNeasy 96 BioRobot 8000 Kit (12) to perform mRNA / Ribonucleoprotein isolation / purification mRNA

Get tips on using MagAttract Direct mRNA M48 Kit (192) to perform mRNA / Ribonucleoprotein isolation / purification mRNA

Get tips on using HT 8-oxo-dG ELISA Kit II to perform DNA Damage Assay Capan-2

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Immortalized cell lines PANC-1

Get tips on using NeuroMag to perform DNA transfection Mammalian cells - Immortalized cell lines SH-SY5Y

Get tips on using OxiSelect™ Comet Assay Kit (3-Well Slides) to perform DNA Damage Assay HeLa

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.