Site Directed Mutagenesis (SDM) Human Deletion K562

Get tips on using CD36 Monoclonal Antibody (eBioNL07 (NL07)), PerCP-eFluor 710, eBioscience™ to perform Flow cytometry Anti-bodies Human - CD36/CB38

Get tips on using Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE to perform RNA isolation / purification Tissue - Human Kidney

Get tips on using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® to perform RNA sequencing Human - MDA-MB-231

Get tips on using Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit to perform Cell cycle assay human - MCF 10A

Get tips on using Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit to perform Cell cycle assay human - PANC-1

Get tips on using Click-iT™ Plus EdU Pacific Blue™ Flow Cytometry Assay Kit to perform Cell cycle assay human - A549

Get tips on using Click-iT™ Plus EdU Pacific Blue™ Flow Cytometry Assay Kit to perform Cell cycle assay human - SKOV3

Get tips on using Pre-designed and validated siRNA against gene IGFBP1 to perform siRNA / miRNA gene silencing Human - Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1)

Get tips on using IMAGEN™ Herpes Simplex Virus (HSV) Kit using Direct Immunofluorescence Assay to perform Cell Culture Contamination Detection Kit Virus

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.