Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.
Get tips on using Dansylcadaverine to perform Autophagy assay cell type - MG-63
Get tips on using RPMI 1640 to perform Mammalian cell culture media HL-60
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized HL-60
Get tips on using RPMI-1640 Medium to perform Mammalian cell culture media HL-60
Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Cells - immortalized HL-60
Get tips on using 1ml NCL-L-Ki67-MM1 to perform Immunohistochemistry Human - Ki-67
Get tips on using APC BrdU Flow Kit to perform Cell cycle assay human - HL-60
Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized HL-60
Get tips on using CD126 antibody | B-R6 to perform Flow cytometry Anti-bodies Human - CD126/IL-6Ralpha
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment