siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells

Get tips on using lenti MS2-P65-HSF1_Hygro to perform CRISPR Human - Activation APOBEC3G

Get tips on using pcDNA-dCas9-p300 Core to perform CRISPR Human - Activation MASPIN

Get tips on using pAC2-dual-dCas9VP48-sgExpression to perform CRISPR Human - Activation IL1RN

Get tips on using UltraCruz® Transfection Reagent to perform CRISPR Human - Activation GRP78

Get tips on using pAC2-dual-dCas9VP48-sgExpression to perform CRISPR Human - Activation SOX2

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using HES1 (D6P2U) Rabbit mAb #11988 to perform Immunohistochemistry Human - Hes1

Get tips on using Notch1 (C44H11) Rabbit mAb #3268 to perform Immunohistochemistry Human - Notch1

Get tips on using Anti-p53 Protein, Clone DO7 to perform Immunohistochemistry Human - p53

Get tips on using CA125 Monoclonal Antibody (Ov185:1) to perform Immunohistochemistry Human - CA125