Get tips on using Anti-Dicer antibody [13D6] - ChIP Grade (ab14601) to perform Immunohistochemistry Human - Dicer1
DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.
Get tips on using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit to perform ChIP Human - Glioblastoma cell line
Get tips on using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit to perform ChIP Human - Fibroblast cell lines
Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Rat - Megakaryocytes
Get tips on using EpiQuik Chromatin Immunoprecipitation (ChIP) Kit to perform ChIP Rat - Colon
Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Rat - NRK52E
Get tips on using Pierce™ Agarose ChIP Kit to perform ChIP Rat - NRK52E
Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Rat - Liver
Get tips on using Pierce™ Magnetic ChIP Kit to perform ChIP Rat - Pancreas
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