Flowcytometry Secondary Antibody Goat Mouse

Get tips on using CD133 (Prominin-1) Monoclonal Antibody (13A4), APC, eBioscience™ to perform Immunohistochemistry Mouse - CD133

Get tips on using CD25 Monoclonal Antibody (PC61.5), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD25

Get tips on using Anti-CD105 antibody [MJ7/18] (PE) (ab93567) to perform Flow cytometry Anti-bodies Mouse - CD105

Get tips on using CD200 Monoclonal Antibody (OX90), PE, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD200

Get tips on using CD73 Monoclonal Antibody (AD2), PE, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD73

Get tips on using CD73 Monoclonal Antibody (AD2), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD73

Get tips on using CD40 Monoclonal Antibody (1C10), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD40

Get tips on using Anti-Prosurfactant Protein C (proSP-C) Antibody to perform Flow cytometry Anti-bodies Mouse - proSP-C

Get tips on using Recombinant Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) to perform Immunohistochemistry Mouse - β-Catenin

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).