Get tips on using pcDNA-oGP1 to perform Protein Expression Eukaryotic cells - HEK293 GP1
Get tips on using pMT3-RhoGC to perform Protein Expression Eukaryotic cells - HEK293 RhoGC
Get tips on using pONE-40A to perform Protein Expression Eukaryotic cells - HEK293 MBP
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Get tips on using pIL-15 to perform Protein Expression Eukaryotic cells - HEK293 IL-15
Get tips on using pFastBac1-TEV-TRPV1 to perform Protein Expression Eukaryotic cells - HEK293 TRPV1
Get tips on using pCMV-HA2/DKK1 to perform Protein Expression Eukaryotic cells - HEK293 DKK1
Get tips on using pgMAX system-DsRed2 to perform Protein Expression Eukaryotic cells - HEK293 DsRed2
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